10 Questions 22 Answers 0 Followers
Questions related from Khawar Sohail Siddiqui
I want to use Glutathione-S-transferase (GST) + monochlorobimane based assay kit that specifically detects GSH in tissue samples such as brain, kidney etc. This is 96-well plate based assay. I...
08 April 2021 4,404 0 View
I am working on the assay of brain acetylcholine. I need an irreversible inhibitor of cholinesterase that is relatively safer (not volatile, preferably solid) to use. This is to prevent the...
09 December 2020 3,904 3 View
we want to grow algae control in the absence of CO2. If we use synthetic air, do we need to continuously bubble it.
09 September 2018 4,618 4 View
I need the exact diameter of ortho silicic acid, Si(OH)4. Kindly provide reference if possible. Thanking in advance for help.
02 May 2018 1,459 4 View
I want to determine which amino acids in a Protein structure are involved in Hydrophobic core. Is there a simple tool which requires a PDB file as input?
09 October 2014 8,979 4 View
My calcium EDTA binding experiments at pH 4.5 and 5.5 using iTC200 in 20 mM sodium acetate are endothermic. The Ca-EDTA kit provided by Microcal gives exothermic peaks in 20 mM MES buffer, pH 5.7....
07 December 2012 5,276 9 View
Want to determine metals in some proteins by ICP. Will be grateful if somebody gives exact recipe for Sample preparation including hydrolysis acid, time, temperature and starting protein amount...
10 October 2012 5,273 1 View
At pH 3.5 to pH 4.5 which is the better and efficient reducing agent to break all 4 disulfide bonds in my peroxidase enzyme? Also which reducing agent and concentration is compatible with far-UV...
01 October 2012 2,436 10 View
Got endotherm of protein only and protein+ ligand. Can any body point to the right Equation to use to determine binding constant from DSC unfolding curves?
10 September 2012 3,423 6 View
I have a set of ~40-50 protein sequences. I want to find motifs like MEME tool but most importantly, I want a tool that can extract a consensus sequence like a scanprosite pattern e.g....
01 January 1970 4,376 8 View
