22 Questions 37 Answers 0 Followers
Questions related from Khawar Sohail Siddiqui
want to visualize SwissDoc protein-ligand docking results in ChimeraX.
23 July 2023 7,230 0 View
I have latest Chimera X version and I want to make an animation without using command line method. first I do not see animation tab under utilities tab. Why? second I am unable to save or encode...
30 June 2023 7,079 0 View
I have to use ice-cold 50 % aqueous methanol to lyse tissue. After centrifugation, the proteins will be in precipitate or in solution or both?
24 February 2022 5,238 0 View
I need to quantify pyruvate levels in tissue samples. However, endogenous enzymes in tissues such as LDH deplete pyruvate. Deproteinization by spin columns is slow and precipitation and...
05 May 2021 4,917 0 View
I want to use Glutathione-S-transferase (GST) + monochlorobimane based assay kit that specifically detects GSH in tissue samples such as brain, kidney etc. This is 96-well plate based assay. I...
08 April 2021 4,471 0 View
I am working on the assay of brain acetylcholine. I need an irreversible inhibitor of cholinesterase that is relatively safer (not volatile, preferably solid) to use. This is to prevent the...
09 December 2020 4,001 3 View
My protein is a heterodimer of two chains produced from a single polypeptide due to post-translational proteolytic cleavage. The templates (x-ray structures) also consists of two chains. How can I...
31 August 2019 9,844 6 View
we want to grow algae control in the absence of CO2. If we use synthetic air, do we need to continuously bubble it.
09 September 2018 4,697 4 View
I need the exact diameter of ortho silicic acid, Si(OH)4. Kindly provide reference if possible. Thanking in advance for help.
02 May 2018 1,594 4 View
Commercially available dialysis tubing and membranes are made up of regenerated cellulose. can these membranes be degraded by cellulase enzymes? I believe that these membranes may be attacked more...
12 November 2017 4,160 5 View
Heme proteins contain charge-transfer (CT) bands. Although CT1 band (637-652 nm) has been assigned to high-spin pentacoordinated state, what the presence or disappearance of CT2 band (near 500 nm)...
17 October 2017 1,473 0 View
During cell division (mitosis & Meiosis), the chromosomes divide. In which stage these duplicated chromosomes becomes individual chromosomes? is it true that any chromatin strucure consisting...
03 April 2016 8,750 4 View
I want to determine which amino acids in a Protein structure are involved in Hydrophobic core. Is there a simple tool which requires a PDB file as input?
09 October 2014 9,042 4 View
I am unable to download EDTSurf from the site. The windows executable file does not run. Is there an online site which takes the PDB file as input and gives appropriate output in form of...
12 September 2014 3,319 1 View
Normally as a result of thermal unfolding the intensity increases and the wavelength of max absorption blue-shifts as Nile Red interacts with the hydrophobic amino-acids. Is it possible that as a...
26 August 2013 781 3 View
My calcium EDTA binding experiments at pH 4.5 and 5.5 using iTC200 in 20 mM sodium acetate are endothermic. The Ca-EDTA kit provided by Microcal gives exothermic peaks in 20 mM MES buffer, pH 5.7....
07 December 2012 5,357 9 View
Want to determine metals in some proteins by ICP. Will be grateful if somebody gives exact recipe for Sample preparation including hydrolysis acid, time, temperature and starting protein amount...
10 October 2012 5,344 1 View
At pH 3.5 to pH 4.5 which is the better and efficient reducing agent to break all 4 disulfide bonds in my peroxidase enzyme? Also which reducing agent and concentration is compatible with far-UV...
01 October 2012 2,527 10 View
Got endotherm of protein only and protein+ ligand. Can any body point to the right Equation to use to determine binding constant from DSC unfolding curves?
10 September 2012 3,494 6 View
When I treat Humic acid with ligninase enzymes, its absorbance at 280 increases. Most references say that absorbance should decrease. What may be the reason?
09 July 2012 7,496 8 View
I have a set of ~40-50 protein sequences. I want to find motifs like MEME tool but most importantly, I want a tool that can extract a consensus sequence like a scanprosite pattern e.g....
01 January 1970 4,457 8 View
I am trying to use web-based TCoffee-Expresso. It is giving me error every time I upload fasta sequences. two are shown below as an example:...
01 January 1970 8,611 0 View
