Trying to choose between a LM or a GLM (family=negative binomial)?

Hi! I have data on captures per year (n=13) and I want to analyze the relation with years (is it increasing, decreasing or staying the same with time). Total captures ~ year Because it is...

23 May 2020 6,009 3 View

How can I compute a factor (latent variable) knowing the variables that contribute?

Dear all, I have a data set with 7 variables. I have run a EFA and I got three factors. I want to obtain a numerical value for each factor computed from the variables that form it but I am not...

20 January 2019 1,933 3 View

How can I compute a factor (latent variable) knowing the variables that contribute?

Dear all, I have a data set with 7 variables. I have run a EFA and I got three factors. I want to obtain a numerical value for each factor computed from the variables that form it but I am not...

31 December 2018 9,034 4 View

How to "define" the terms "chemical experience" and "chemical intuition"?

Chemists often use terms like "chemical intuition" and "chemical experience", but I'm curious to know what do they mean to the chem community. How coudl you put them in a few words?

10 November 2016 4,388 0 View

How can I substitute a LEU residue with an ALA one using Modeller?

I'm trying to mutate a LEU residue in a peptide, using Modeller, but the final PDB is the same that the starting one.

01 February 2016 1,515 3 View

Does anyone has experience with the IEC-18 cell line?

We have problems in forming the monolayer of IEC-18 cells, even when protocols in literature are rigorously followed . Could anyone give some tips for achieving a good monolayer formation? Thanks...

09 October 2015 4,529 2 View

How can I distinguish irreversible inhibition from noncompetitive inhibition since both have lower Vmax and unchanged Km?

I have come accross this tricky question and could not find a suitable explanation anywhere. Since both abovementioned types of inhibition show the same pattern, how can I make sure which one I am...

28 May 2014 7,575 4 View

What software can I use to compute the conceptual-DFT Fukui function?

Im interested in the Fukui function of conceptual-DFT, but I dont know how to compute it.

31 December 2012 7,301 15 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View