Hello Victoria Quintana

Molecular weight of Ouabain is 584.6525 g/mol

Therefore 1M Quabain = 584.6525g in 1Litre solution.

So for 1 uM Quabain = 584.6525ug in 1Litre solution.

Therefore to make a 100nM Quabain solution in 1 Litre

584.6525ug x 100 nM / 1000nM = 58.46ug in 1 Litre

Which is 5.846 ug in 100ml solution.

If you are using Ouabain octahydrate, the molecular weight is 728.77 g/mol

Then in this case it will be 7.2877 ug in 100ml solution.

Best.