Nai-Kei Wong

3 Questions 116 Answers 0 Followers

Questions related from Nai-Kei Wong

How to measure bacterial membrane potential by fluorescence measurement or imaging?

I'm interested in using fluorescence dyes to measure or image bacterial (Gram negative) membrane potential. I have tried PI (propidium iodide) before, but wonder if there are better methods or...

11 November 2018 3,423 2 View

Which LPS would you consider "most typical" and suitable for testing in human and murine cells?

Hello, I am interested in testing the various effects of LPS in in vitro models of human (immortalized cell line) liver and mouse liver cells. I want to use an LPS that is enterobacterial and...

03 March 2015 1,012 7 View

What heat killing conditions do you use for bacteria?

Often a heat-killed version of the bacteria is used, to contrast with live bacteria in infection assays. There are various different conditions being used in the literature, ranging from 50°C to...

03 March 2015 8,084 9 View