You could use: human mammary epithelial cell line HMEC (American type culture collection (ATCC): HTB-30)

Hi Danijela,

ATCC has a wide collection of cell lines from various species and organs. I searched its catalogue for human breast cell lines for example, you can browse it on this link:

http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/tabid/961/Default.aspx?CollectionID=106

Have a nice day :)

Csaba

Ooops, there're some glitches in the link, use these keywords in the ATCC search: mammary gland homo sapiens :)

Thank you very much!

More cell lines, Cat nº ATCC: CRL-8798; CRL-8799;CRL-10783

You could by in this page

http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx

what about MCF-7?

It depends on whether you want ER responsive or not and whether you are planning to undertake in vivo experiments. You could try MCF-7.

MCF10A and HLB-100

MCF7

Thank you very much! :)

MCF-7 this a sensitive breast cancer (ductal carcinoma) cell line. Sometimes called wild type. I hope I ahve answered your question, They are sensitive to antihermonal therapy & chemotherapy as well. If you have difficulty in getting them, please let me know. MDA-MB-213 they are breast cancer (ductal carcinoma) cell line. However, they are ER -ve (not responding to Tamoxifen).

Do you mean a normal benign epithelial breast line or a breast cancer line? There are many subtypes of breast cancer and lines that reflect those subtypes. I am not sure what you mean by normal cell line.

Do you mean non-tumorigenesis breast cell lines? MCF12A and MCF10 are lines from breast, but non-tumorgenesis. Those lines can used as control and you can get from ATCC.

MCF-7 is Breast cancer cell line (adenocarcinoma) - ductal carcinoma

Your r welcome, let me know if you need further info

Hi Danijela

Here are a couple of papers that may be of use. The first is a simple overview of breast cancer cell lines and their usefulness:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3236329/?tool=pmcentrez

And the second profiles most of the common breast cancer cell lines (including MCF10A's).

http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0006146

Also, I would recommend getting your cells from a reputable biorepository such as ATTC (http://www.atcc.org/) or ECAC (http://www.hpacultures.org.uk/collections/ecacc.jsp) instead of working with someone else's dubious cultures.

Finally, be aware that many breast cell lines are not actually 'breast'. MDA-MB-231's for example are commonly used to represent triple negative breast cancer, however these cells were derived from a pleural effusion (ie not the primary lesion) and exhibit mutations almost never seen in breast cancers (eg BRAF mutations). So be wary :)

Hı,

we mostly use ER (+) breast cancer cell lines as MCF-7 and we gather them from ATCC. We are in Turkey and they usually arrive within 4-6 weeks time...

But apart from that if you want to work with normal breast cells, those are different, one example for that is MCF 10 A... You can use them as controls for your experiments...

Good luck...

Thank you very much! :)

Here is another paper that documents a panel of breast cancer cell lines and molecular profiles, etc very similar to what Alasdair posted, but a little larger panel... Hope this helps

http://www.ncbi.nlm.nih.gov/pubmed/17157791

Thank you:)

Yes, I think MCF-7 cells (ER+, PR+) would be a good complement to the cell lines you already have, maybe together with MCF10A cells if you also want to study non-malignant cells (for example, if you want to study drug selectivity). T-47D is another hormone-dependent cell line which is applied very often.

Thank you! :)

Thank you very much! :)

To complete the MCF10A cells, there are Ras-transformed variants MCF10AneoT (transformed non-invasive) and MCF10CA1a (transformed and invasive) that you can get from the Karmanos Cancer Institute, Detroit, USA (formerly the Michigan Cancer Foundation where the MCF cell lines were generated originally). They may represent a nice model of "normal" vs tumor breast cell lines, with isogenic background.

Beware the HBL-100 cell line, chromosome Y is detectable (kind of sad for a female breast cell line :)

Karyotype info from: http://www.cell-lines-service.de/content/e3969/e4254/e4265/index_ger.html

The stemline chromosome number is near triploid with the modal number of 67 chromosomes, and the 2S component occurring at 0.6%. Most chromosome complements consist of about 39 normal and 28 marker chromosomes. Markers such as 2q, 11q+, 11q, t(2q;12), t(2q;5q?), t(6p?;16), 16pt and many others are common to most metaphases. Normal chromosomes 11, 14, 15 and 16 are absent; 2, 12, 17 and 19 are monosomic, and the X is disomic.

NOTE: According to reports by ATCC, DNA profiling for amelogenin, a sex-chromosome-specific PCR assay that can distinguish X chromosome-specific products from Y chromosome-specific products revealed the presence of Y chromosomes in this cell line of putative female origin. Confirmation of the general findings was accomplished by QM staining, C-banding, and FISH, with a whole chromosome paint probe to the human Y chromosome.

MCF-10A and MCF-12F are the two most common normal immortalized cells. We usually buy from ATCC

Thank you:)

Thank you:)

Remember that "normal" cell lines such as MCF10A or any other immortalized HMECs have a basalish/myoepithelial phenotype that is far away from being luminal/ER+....

MCF10A might be a good option for normal breast cells. ( these are not cancer ...) We always buy cells from ATCC, it may be a little bit expensive but they are really reliable and the cells reach on time in your hands and they are healthy...

Good Luck..

Burcak

If you want a 'normal' breast cell, purchase untransformed mammary epithelial cells from Lonza. These cells are isolated from patients who have breast-reduction surgery. Use them for about 6 passages only (you can expand and freeze them if you've got a lot of experiments to do). They require MEGM (-2) to grow. In my experience, MCF-10A cells act much more like a cancer cell than these do. http://www.lonza.com/en/products-services/bio-research/primary-and-stem-cells/human-cells-and-media/mammary-epithelial-cells-and-media.aspx

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Also try life line cell technology, they sell several normal *(primary) cells initiated from various human tissues.

you can choose MCF-10 cell line as normal breast cell

HMEC is best. MCF10A and MCF10F are both transformed lines, and non-tumorigenic, so useful as controls for tumorigenicity, and other malignant cell phenoptyes. MCF7 is a hormone responsive carcinoma cell line (not phenotypically or molecularly comparable to normal breast epithelial cells. All cell lines mentioned above are available from the ATCC. Best of luck.

Parham,

thank you very much. I am just dealing with the drug resistance test in these two types of cancer.

All the best