Hi -

In the paper by Singh et al in Cancer Research (2000) it would appear to be possible to grow these cells in DMEM. It can be found via the following link:

http://cancerres.aacrjournals.org/content/60/12/3305.full.pdf+html

I hope this helps

Best regards

Alan Hargreaves

I guess tha my response is actually too late for you but may be it could be of help for other researchers.

I have serious doubts about the fact that once a given cancer cell line has been established, the researcher(s) who established this cell line has / have tested all the media / sera routbiley used in labs.

Thus, if any researcher wants to "deviate" from a "given publication" about a "given cell line" and about a "given medium + serum", she/he has just to test it, to look at the cells under a phase-contrast microscope and to establish the Gomperz-related curve in the medium + serum of "reference" as compared to the "new" conditions she/he has decided to use ... and to precisely describe them in the M&M of her/his article.

I am old enough to know "today" that everything that is reported in the "scientific" literature is not always the "absolute truth" ... at least from a scientific point of view ...

and above all dear Danijela and "others", science needs sometimes some deviations from "standard protocols" to perform significant achievements ...

Best regards

Robert

I used alpha MEM medium with 10% FBS, 1% Pen-Strp, 2% Hepes(1M ), 1% 100x non-essential amino acids( 10mM) , 1% Na-pyruvate( 100mM) , 1% L-Glutamine , 0.1% 1000x2-ME ( 55mM). It works great for MDA-MB-468.