Asma Future

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Questions related from Asma Future

What are the reasons for giving different values of CT in triplicate when using SYPR green?

i extracted RNA from MCF-10 by trizol , and ct of cDNA of MCF-10 of LKB1 gene gave strange value ..cDNA diluted 1:4 ,ct value with GAPDH 11 in triplicate, but with interested gene ct values...

08 April 2015 1,660 2 View

If a protein works in the cytoplasm, after the synthesis in cytoplasm by ribosome, does it remain in the cytoplasm or go back to the nucleolus?

my protein LKB1 work mainly in cytoplasm and work less in nucleus, and it has nuclear localization signal and other protein (STRD alpha and MO25)to help to transfer it from nucleus to cytoplasm...

01 April 2015 9,404 3 View

Does gene expression of any gene in breast cancer line reflect the gene expression in vivo in the breast ?

when we dealing the cell to convert to cell line , the property of the cell line like gene expression of cell or phenotype from the vivo tissue ? do we can depend the result expression of gene in...

30 March 2015 7,964 8 View

Why did I not got any results with lkb1 primer in real time pcr?

I had mcf-10 cell and was grown in dmem/f12 media with fbs and AB and rna and dna was extracted from the cell ,, when I did RT-PCR with cDNA , I got results with GAPDH but not with my interested...

19 January 2015 9,858 3 View

Why the different size of band with the same gene in pcr ?

I have rna for MCF-10 and MCF-7 ....the concentration of rna of mcf-7 is 884 ng/microliter with 260/280 1.76 .. the concentration of rna of mcf-10 is 668 ng/microliter with 260/280 1.91 I convert...

07 January 2015 8,924 7 View

If I isolate DNA and RNA from cell monolayer culture by Trizol, do I need to centrifuge after adding Trizol and before adding choloroform?

My cells are MCF-7 and MCF-10. I want extract DNA and RNA from these cell using Trizol. Do I add Trizol directly into the flask after removing the media? What amount of Trizol is needed if I grow...

07 December 2014 7,073 5 View

What is appropriate media for MCF-7 cell and MCF-10 ?

I have mcf-7 and mcf-10 and I grow them in DMEM 12 media. Is this a suitable method?

11 November 2014 9,926 1 View

What are the reasons for no amplification in RT-PCR?

When I extracted total RNA from cell (tumor and normal) and I made one amount of RNA to convert to cDNA, I got no reverse transcription reaction. I diluted cDNA to 100 microliters, in addition to...

25 April 2014 3,770 3 View

I have MCF-7 and MCF-10: what is the difference between them?

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07 February 2014 5,211 3 View