Why does a particular point mutation serine to alanine lead to significant change in protein mobility on SDS-PAGE?

I made several point mutations on a protein, and found that only one particular Ser to Ala mutation leads to significant change in mobility of protein on SDS-PAGE (WB is attached). The drop in...

16 January 2020 3,661 4 View

Change in protein mobility on SDS-PAGE when Ser is mutated to Ala?

I made several point mutations on a protein, and found that only one particular Ser to Ala mutation leads to significant change in mobility of protein on SDS-PAGE (WB is attached). The drop in...

16 January 2020 8,844 4 View

Neural Networks in SPSS anyone?

Im analyzing the impact of different biomarkers and clinical risk scores on postoperative complications and Id like to test the importance that every factor has on the final outcome. Even if I add...

12 February 2018 2,701 6 View

Adjusting results in SPSS

I need to adjust my biomarker results with comorbidities, age and sex. Hopefully I will be able to enter the results into the ROC curve and compare it to original ROC curve (without adjustment). I...

28 May 2017 1,664 2 View

Covariate adjustment in SPSS

How can I perform covariate adjustment in SPSS (or AROC)? Seems that there are no guidelines, the only thing I can find are theoretical explanations which are not quite clear. Thank you in advance.

23 May 2017 6,110 8 View

Does anyone know how acetone or ethanol effect Ca-alginate beads?

Ca-alginate beads are effective drying beads, but I was wondering if they can change anything in the gel structure?

20 January 2013 5,367 2 View

Can I use DMEM for cells cultivation, MDA-MB-468?

It is triple negative breast cancer cell line. Thank you for your help

07 November 2011 2,087 3 View

Weird biosorption isotherm data

Hello everyone... I was trying to study the biosorption process of Pb onto bean shells, and I got this type of isotherm data... I have a problem fitting it, because no model seems to fit my data...

01 January 1970 5,639 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View