Does the N-terminal portion of LH/hCG-R get cleaved following trypsinization of cells?
I'm a little confused, I am doing a PCR reaction that requires 100ng of genomic DNA template, and the concentration of my genomic DNA is 591,9ng/ul. So is 100ng of the template the same as saying...
25 February 2023 9,445 3 View
Hello, dear colleagues from the world of science, I have recently started investigating the process of copper ions biosorption using eggshells as an adsorbent. I have studied multiple...
13 October 2022 8,983 5 View
Hi CROPWAT users! Lately, I have had a problem with entering the sowing date in the CROPWAT model. No matter what date format I enter, the model response is always the same - invalid date (date...
23 January 2022 4,332 2 View
I am critiquing a paper that has done a case study of 5 systems (4 of them in java) and their domains are: embedded systems (2 of them), IDE (Netbeans), and whatever JRefractory is, some sort of...
04 September 2020 3,125 3 View
I made several point mutations on a protein, and found that only one particular Ser to Ala mutation leads to significant change in mobility of protein on SDS-PAGE (WB is attached). The drop in...
16 January 2020 3,820 4 View
16 January 2020 8,985 4 View
I have been using surface modified natural zeolites for the removal of diclofenac and ketoprofen. As isotherm models, I have tried Langmuir, Sips and Toth isotherm model. In general, the last two...
20 October 2019 3,960 3 View
Can somebody please help me to manipulate a spoken utterance to sound like the one presented on https://www.theatlantic.com/technology/archive/2014/06/sounds-you-cant-unhear/373036/?
06 May 2018 6,246 3 View
Im analyzing the impact of different biomarkers and clinical risk scores on postoperative complications and Id like to test the importance that every factor has on the final outcome. Even if I add...
12 February 2018 2,850 6 View
I need to adjust my biomarker results with comorbidities, age and sex. Hopefully I will be able to enter the results into the ROC curve and compare it to original ROC curve (without adjustment). I...
28 May 2017 1,911 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View