Christiana Neophytou

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Questions related from Christiana Neophytou

Does anyone have a protocol for measuring anti-estrogenic activity in Ishikawa cells by using an alkaline phosphatase substrate (P4744 by Sigma)?

I want to determine whether some novel compounds can interfere with estradiol activity by using this Alkaline Phosphatase activity assay in Ishikawa cells.

19 June 2015 104 0 View

Is it possible to have inhibition of DNA fragmentation with z-vad-fmk but not cleavage of PARP?

I am working with a synthetic compound that can induce DNA fragmentation in MCF-7 and MDA-MB-231 cells. When I co-incubate with z.vad.fmk, fragmentation is only partially inhibited in MCF-7 cells...

24 April 2013 2,584 6 View