I want to perform WST-1 test with alginate based 3D-colorectal cancer cells. I wonder about before performing WST-1 test, ıs it necessary to remove alginate spheres from the cells (to carry it from 3D to 2D 96 well-plate)? or can ı perform the test with 3D alginate spheres? Thank you for your contribution.
You can perform the WST-1 test directly on alginate-based 3D colorectal cancer cell cultures without removing the alginate spheres. The WST-1 reagent can penetrate the 3D structure, allowing the assay to measure cell viability within the 3D environment. Here are some tips:
1. **Ensure Adequate Reagent Penetration**: Mix the WST-1 reagent thoroughly with the medium to ensure it can diffuse into the alginate matrix.
2. **Incubation Time**: Allow sufficient incubation time for the reagent to react with cells within the 3D structure.
3. **Readout**: Measure absorbance as per standard WST-1 protocols.
This approach allows you to maintain the 3D culture conditions and assess cell viability more accurately in the 3D context.
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