What the error mean square (in a split plot design) is always higher for the main plot error as compared to the sub plot error ?

I know that in split plot design the main plot error is larger than the sub plot error. There is more error degree of freedom for sub plot as compared to main plot. But, in statistical analysis,...

14 December 2020 6,669 4 View

Which are the best methods used for isolation and purification of plant pathogenic fungi and bacteria ?

Best methods suitable for isolation and purification of plant pathogenic fungi and bacteria from plant and soil samples....Latest methods....Any special advantages....Procedure to be followed...etc.

28 November 2020 3,365 3 View

How health psychology is helpful to deal the daily life issues?

Psychological Impact of Health Disease on Human Behaviour. If anybody is interested they can join us.

10 August 2020 8,007 13 View

Does plasma therapy works for treatment of covid-19?

04 August 2020 8,186 11 View

What is the correct way to do the formulation of engine oil based nanofluid?

When we apply Tiwari-Das model of nanofluids to model the flow of engine oil based nanofluid, should it be done using the term which denotes relaxation time for velocity in momentum equation...

30 March 2020 8,383 2 View

Any researcher working on Alkaloids in India?

Any researcher working on the alkaloids(Trichodesmine, Junceine, Riddelline, Senecionine, Seneciphylline, Lycopsamine and Intermidine) in india. Need help regarding this.

03 March 2020 1,060 4 View

In ansys CFD, simulation, Can we initialize two fluids kept in two adjacent compartment with two different temperatures?

Compartment wall temperature can easily initialzed with boundary conditions but if fluid inside it are at different temperatures?

03 February 2020 4,495 4 View

In Ansys CFD can we assign cell zone temperature conditions along with existing fluid/solid material conditions?

Cell zone conditions are about assigning a particular fluid or solid inside it, but it does not allow hot or cold conditions of fluid inside it. can we assign them in Ansys CFD simulation?

03 February 2020 5,199 4 View

How do we determine the position and orientation of a moving robot ??

How do we determine the position and orientation of a moving robot using IMU sensors such as MPU6050 or MPU9250 (i.e. accelerometer , gyroscope and magnetometer sensor data)??? kindly advise...

22 November 2019 7,825 5 View

Why overaging treament is required in continuous annealing line for production of dual phase steel?

Dual phase steel can be produced by directly quenching to room temperature from inter-critical temperature range. Then why overaging treatment is given in continious annealing line in steel...

09 September 2019 6,322 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View