10 Questions 12 Answers 0 Followers
Questions related from Ravindra Singh Rawat
Hi everyone! I have been trying to run my simulation on BRAF server. However the script I am loading is not working. It shows the following error: 'Sbatch : error: This does not look like a batch...
01 August 2021 3,785 3 View
Hi everyone My protein has a molecular weight of 40 kDa. I have been trying to crystallize my protein and has not been able to observe any crystal but only precipitate in any screen. I have used...
20 December 2019 3,593 3 View
Hi everyone I have been trying to resolve adenosine and Inosine on HPLC column. The details are as follows: 1) Column dimension: 150mmX4.6mm, with 5 micron bead size. 2) Mobile phase: 20mM...
26 August 2019 3,556 3 View
Hi everyone! I have designed a peptide which has a beta hairpin loop structure. I have mutated one of the residues of the peptide in order to check if mutation leads to change in beta hairpin...
16 November 2018 6,314 5 View
Hi everyone! I am trying to express a bacterial protein which I have cloned in pet28a+ vector with NcoI site and XhoI site with C-terminal his-tag. The bacterial host I am using is BL21DE3 plysS....
01 April 2018 4,662 4 View
Hi everyone! Could anybody please suggest me, where to get CH184 E.coli strain from? This strain is streptomycin-pseudo-dependent strain. If somebody has it, would it be possible to kindly...
29 March 2017 6,406 2 View
Hi! everyone I am trying to clone a specific gene directly from bacterial chromosome.regarding which I have following questions: 1) Is it correct to PCR amplify gene directly from chromosomal DNA...
06 July 2016 5,478 7 View
Hi everyone Does sodium deoxycholate solubilizes protein from inclusion bodies?
08 March 2016 2,904 2 View
Hi! everyone Is it possible to lyse bacterial cell using Sodium dodecyl sulphate in lysis buffer without denaturing proteins?
02 February 2016 9,117 4 View
Hi everyone! I have cloned gene in pRSETB vector and expressed in BL21(DE3) host. After fresh transformation protein is expressed. However, there are following difficulties: 1) Even only one week...
01 October 2015 5,658 11 View
