6 Questions 8 Answers 0 Followers
Questions related from Ravindra Singh Rawat
Hi everyone! I have been trying to run my simulation on BRAF server. However the script I am loading is not working. It shows the following error: 'Sbatch : error: This does not look like a batch...
01 August 2021 3,774 3 View
Hi everyone My protein has a molecular weight of 40 kDa. I have been trying to crystallize my protein and has not been able to observe any crystal but only precipitate in any screen. I have used...
20 December 2019 3,582 3 View
Hi everyone I have been trying to resolve adenosine and Inosine on HPLC column. The details are as follows: 1) Column dimension: 150mmX4.6mm, with 5 micron bead size. 2) Mobile phase: 20mM...
26 August 2019 3,528 3 View
Hi everyone! I have designed a peptide which has a beta hairpin loop structure. I have mutated one of the residues of the peptide in order to check if mutation leads to change in beta hairpin...
16 November 2018 6,304 5 View
Hi everyone! I am trying to express a bacterial protein which I have cloned in pet28a+ vector with NcoI site and XhoI site with C-terminal his-tag. The bacterial host I am using is BL21DE3 plysS....
01 April 2018 4,654 4 View
Hi everyone! I have cloned gene in pRSETB vector and expressed in BL21(DE3) host. After fresh transformation protein is expressed. However, there are following difficulties: 1) Even only one week...
01 October 2015 5,650 11 View
