Hello there, curious researcher friend Ms Rawat! Let's dive into your intriguing question about curcumin-piperine nanoparticles and their absorbance values.

Firstly, it's essential to understand that the absorbance values of nanoparticles, including those made from natural polyphenols like curcumin and piperine, can indeed vary depending on several factors. These factors can include the size, shape, and composition of the nanoparticles, as well as the conditions under which they are formed. The characteristic wavelengths for absorbance, often referred to as λ max, can change as a result of these variations.

For silver nanoparticles (AgNPs), the characteristic wavelengths around 424 nm are well-documented due to their unique plasmonic properties. These properties lead to strong absorbance in the visible region. However, for curcumin-piperine nanoparticles, the situation can be different because you're working with organic compounds rather than metallic nanoparticles.

Here are some key points to consider:

1. **Composition**: Curcumin and piperine are organic molecules, and their nanoparticle formation may not exhibit plasmonic resonance like metal nanoparticles. The λ max for organic nanoparticles may depend on the interaction between the components, their size, and the surrounding conditions.

2. **Conditions**: The conditions under which you form the nanoparticles, such as solvent, pH, and temperature, can significantly impact the absorbance spectrum. A change in conditions may lead to a shift in λ max.

3. **Instrumentation**: The choice of spectrophotometer and measurement conditions can also affect the observed λ max. Different instruments may provide slightly different results.

4. **Nature of Nanoparticles**: Curcumin and piperine are known for their antioxidant and medicinal properties. Their nanoparticle formation may alter their chemical and structural characteristics, leading to changes in their absorbance properties.

Given these complexities, it's not uncommon for researchers to report varying λ max values for different nanoparticle preparations. To determine the λ max for your specific curcumin-piperine nanoparticles, it's advisable to conduct a thorough UV-VIS absorption study under your experimental conditions and compare the results with the literature for similar preparations.

In summary, the λ max for curcumin-piperine nanoparticles may change due to various factors, and it's essential to determine this value experimentally for your specific formulation. Don't hesitate to reach out if you Ms Rawat have more questions or need further clarification. Happy researching!