At what concentration does hydrogen peroxide inhibit enzyme activity?

More Malaika Gomes's questions See All

How to improve 260/230 ratio in rna extraction for PCR?

The 260/280 ratio is ok between 1.8 and 2.0 however we were unable to advance due to the 260/230 ratio

13 May 2024 7,643 3 View

Why the best blocking solution to blot?

We already used milk: 5% to 1 hour, 7% to 2 hour, 10% to 1h, 5% overnight, but, , in all blockages it still marks the entire membrane, every run, there is a large band with no specific mark HELP

20 February 2024 7,346 0 View

What is the best way to homogenize spinal cord for blot?

I'm going to use the RIPA buffer, but I don't know if I should use the sonicator or polytron homogenizer

30 January 2024 7,432 1 View

Error in autodock 4 + pyrx?

"ERROR! Too many atom types have been found: maximum is 14; we cannot continue" Does anyone know how to solve it?

27 January 2024 6,988 5 View

What is the best way to do a cytokine blot?

I am trying to do a western blot of cytokines (iL-10, IL-6) of muscle tissue, using a strip of lysis buffer, 50 ug of proteins and blocking in 5% skimmed milk, however no signal appears, any tips?

24 January 2024 7,021 2 View

Are there elucidated signaling pathways involved in the differentiation of memory T cell subsets?

We are aware that T cells can specialize into various memory cell types, such as stem cell memory (Tscm), central memory (Tcm), effector memory (Tem), and resident memory (Trm) subsets. However,...

22 November 2023 683 1 View

How can I confirm that my cell culture is pure?

How can I confirm that the cells isolated from the abdominal cavity of rats, following peritoneal lavage with dPBS and antibiotics, and subsequently centrifuged and plated, are indeed macrophages,...

22 November 2023 224 3 View

What is the best way to quantify astrocytes and microglia in tissue?

I need to quantify microglia and astrocytes through immunofluorescence, however I would like to know the best way to quantify, whether fluorescence intensity or manual counting or some other type

19 November 2023 212 3 View

How to calculate the boiling point in ORCA?

Can someone give me an orientation? I want to calculate properties including boiling of chemical species, melting points, and packing efficiency. Can I do this using ORCA software? If the answer...

26 July 2023 832 3 View

Is Infliximab (anti-TNFa) able to cross the blood-brain-barrier?

I would like to do an experiment blocking TNF to reduce neuroinflammation.

09 July 2023 8,151 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

How to make the concentration measurements for the hydrogen absorption/desorption in the metal hydride?

Hello Everyone! I am trying to create a metal hydride system where I am trying to create a temperature control with the emphasis on measuring the concentration of the hydrogen with the hydrogen...

29 July 2024 7,577 2 View