Kindly let me know if i can autoclave trypan blue solution as I wish to add it into Agar plates to see clear zones for cellulase activity.
I also have to add CMC to the agar plates, so is 1% CMC solution autoclavable?
Hi Goutham,
I have't used Trypan blue personally. However, I have seen some papers where they use established protocols which mention to use 121 degree autoclaving (on a gentle cycle). However, I have used CMC in my protocols. The polysaccharide has been mentioned to survive at 121 degree autoclaving. However, we generally autoclave this material at 115 degrees for 10 minutes (gentle autoclaving cycle) as we have seen noticeable (although, minor) amounts of hydrolysis on 121 degrees.
Hello. I don't autoclave my trypan blue, as I only used it as a dye. However, I do autoclave CMC.
Autoclave CMC, but in pure water (no divalent cations). CMC solutions are too viscous to be filtered.
Thank you Nurul and Pierre.
Mr. Avinash I wish to know if u were mentioning regarding trypan blue autoclaving in the initial sentences.?
And have any one tried to autoclave trypan blue. as i am supposed use it for screening and add to LB agar and incubate for 48 hours i wish that my trypan blue is sterile. so what would you recommend to just use 0.2 micron filter syringe or to autoclave. If u can take some time kindly attach the article where they have autoclaved trypan blue.
Thank you.
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