How can one calculate the copy number with following details:
plasmid size 4000 bp
concentration 1600 ng/ul
Now I did try using thermoscientific online tool which does tell u total copy numbers per ul with above details.
but I find the tool is misleading the calculation.
for example: using above values the tool gives me the number of copies per ul right. (eg: if it says 3X10^11 copies/ul)
Now if I wish to make a copy number of 50 copies/ul in 10 ul it shows that I need to add ~1.2 ul + 9.9 ul , now If i raise my copy number to 500 copies/ul in 10 ul it says the same 1.2+9.9? how.?
Other strategy which I guess most people follow : from the main stock copy number calculate it for 1X10^8 and then make serial dilutions to bring it to 5 copy number?
Which is the better option?
Application is to use the plasmid copy numbers for standard curve preparation to determine viral dose in clinical samples.
Please share your experiences and calculation methods/tools
You can calculate the copy number for your plasmid from the link https://cels.uri.edu/gsc/cndna.html
You just enter the amount of DNA (ng):
and length of template (bp)
Since both of the required field you know, you will calculate copy number from the above link easily
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