Hello everyone.
I'm trying to form spheroids fom hct116 cells. The first time I did the experiment I could see a defined edge around the spheres and they looked tight. Now all I get are those agregates in the picture. Are those true spheroids? I'm sorry for the picture quality, it was the best I could take.
Thank you!
P.S: Here is the protocol I use:
- I coat the wells of a 96 well flat bottom plate with 50 uL of agarose 1.5%;
- I seed 800 cells per well in 200 uL of medium;
- Centrifuge 1000xg for 10 min;
- Incubate for 4 days.