Gabriel M. C. Longo

4 Questions 5 Answers 0 Followers

Questions related from Gabriel M. C. Longo

Wnt pathway not upregulated on CRC cell lines harboring mutations in APC and Beta-catenin?

Hi!I am currently studying a novel compound in CRC treatment. We see cell death induction when we treat the cell lines (HCT116 and HT29) and we wondered if the wnt pathway could be modulated by...

15 April 2017 5,710 2 View

Are these true spheroids?

Hello everyone. I'm trying to form spheroids fom hct116 cells. The first time I did the experiment I could see a defined edge around the spheres and they looked tight. Now all I get are those...

07 March 2016 7,836 3 View

How can I determine a clinically relevant concentration?

Hi! I am testing a new compound on colorectal cancer cell lines and I want to compare it's efficacy with the standard drug utilized in the treatment, which is the 5-FU. What concentrations should...

26 December 2015 9,762 4 View

Cells treated with DMSO show less viability than cells treated with the compound, how is that possible?

I have performed a MTT viability assay to see how my cells (MCF-7 and HCT-116) respond to a drug. I had one control with cells + medium and another control with cells + medium + DMSO in different...

29 August 2015 2,272 14 View