Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is worth buying a real-time pcr, since an endpoint pcr is sufficient for our objectives.
If your immediate needs are endpoint pcr then I would look into whether you can get discount for buying 2 machines and get a gradient pcr machine as well as a simple end point pcr machine . They can both be used for simple pcr and the gradient machine will save a lot of time setting up new primer sets as your work diversifies. The maintenance on end point pcr machines will also be cheaper than the rtpcr machine.
If your immediate needs are endpoint pcr then I would look into whether you can get discount for buying 2 machines and get a gradient pcr machine as well as a simple end point pcr machine . They can both be used for simple pcr and the gradient machine will save a lot of time setting up new primer sets as your work diversifies. The maintenance on end point pcr machines will also be cheaper than the rtpcr machine.
Thank you all for your comments, they have been very useful. We finally decided to buy a gradient endpoint PCR. We believe that it will be enough for our goals
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