Recently I've been doing some PCRs and the primes used to work a month ago, but now that we want to repeat the experiment, they are not working anymore. I have noticed something in my gels, and it is that I dye them with ethidium bromide and then wash the excess with distilled water. When I reveal the gel in the transilluminator the DNA seems to faint until it disappears. I immerse the gel in the EtBr solution again but to no avail, it's like the DNA is leaving the gel somehow. Anyone knows wats going on?

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