I have read a lot of papers reporting somatic embryogenesis of Theobroma cacao, however only a few embryos are obtained and the procedures are poorly reproducible. On another hand, micropropagation via direct organogenesys are not reported.
Los protocolos utilizando Embriogenesis somatica para propagar cacao son totalmente reproducibles y hay mucha literatura sobre ello. La ES es altamente dependiente del genotipo, pero en genotipos con baja reactividad embriogenica, es posible obtener mayor cantidad de embriones haciendo embriogenesis secundaria. Existe mucha informacion publicada de actividades de ajuste de la tecnologia efectuadas en PSU, NESTLE, CIRAD/Francia, INIAP/Ecuador....etc. La organogenesis directa, generalmente no permite resultados esperados en terminos de conformidad morfologica y genetica en cacao.
Dear Isidro,
I have no experience with Theobroma cacao somatic embryogenesis (SE) or organogenesis. However, I have dealt with this troubling issue (reproducibility of results in plant tissue cultures) in other species for many years. Here I will briefly make one point in general terms.
Compare the protocols of papers describing Theobroma cacao SE. Significant differences between these protocols indicate that no standard protocol served different research groups. Probably, later research groups could not reproduce (to their satisfaction) the results with the protocols developed previously. If this is the case, you will likely need to select one protocol as a starting point for your approach to refine it to suit the Theobroma cacao genotype(s) you are working with. As unfortunate and disappointing as it is, this is the accepted practice...
Best wishes,
Benjamin
If the availability of the previous studies is less. Testing range of PGRs con. and combinations with multiple growth conditions would be the best choice. This is the only way to optimize your reproducibility.
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