Can anybody help me to interpret electropherograms dna sequencing?

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Why anthocyanins from my extracts are not being detected?

A sample rich in anthocyanins has been passed through UHPLC-DAD, using a gradient with acidified water (formic acid 0.1%) and methanol and a flow rate of 0.5 mL/min. However, the chromatogram is...

11 June 2023 3,935 3 View

Is there someone from Mexico who would like to donate 1g of tetrazolium chloride please?

I wanna do an experimental assay to determine seed viability. Thanks

01 March 2023 6,950 4 View

What kind of filter can be used for aqueous samples for GPC?

I want to analize a sample of water soluble polymer but i want to know the type of filter can be used for my sample

30 June 2019 8,368 2 View

Can you gel purify promoter+gRNA ONLY (i.e. no backbone) for RNA synthesis (CRISPR Cas9 gene editing)?

gRNA kits say to "linearise" the plasmid containing your promoter+gRNA right after the termination signal. I was wondering whats the point of having the whole backbone there?. Cant you just cut at...

07 May 2015 1,084 3 View

How should I manage my SSR data: qualitatively or quantitatively?

I want to correlate quantitative traits with SSR markers data, using Pearson method. How should I manage my SSR data: qualitative or quantitative? Thanks for your peer comments

07 August 2014 7,821 3 View

How can I put a blunt-sticky ligation of 6.3kb insert into 2.3kb backbone?

Can anyone give some advice on my cloning? This is probably not the situation that you want to have where your insert is almost 3 times larger than your backbone. I tried several times now. I use...

01 July 2014 8,706 14 View

Is it possible to detect and quantify terpenoid compounds using gas chromatography?

I am working with extracts of different parts of the plant Jatropha curcas trying to detect the terpenoid molecule jatrophone. All reported protocols use HPLC to detect jatrophone, but I don't...

29 December 2012 5,586 26 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View