I am using a specific brand of MS Basal medium with vitamins and I see that my plants are not growing as fast as they should. I am attaching the medium composition of the brand I am using and the one I would like to try. Any recommendations?
Dear Giorgios, try to add some coconut water. You will get faster shoot growth.
Best Reagards,
Pratik
Dear Georgios Formozis every formulation has its own composition: that's why it has a name, in such a way that every researcher using the same formulation are using the same composition. Therefore, in this case, as much as you can say is that you're using a modification of MS. With the few details you're providing, is difficult to make any suggestion. Mineral composition of medium is esential to provide to the in vitro materials suitable conditions for growth. However, other factors are also critical for that: homones, size and kind of explant, carbon source, inoculum density, volume of culture medium, lenght of subculture, culture conditions....
Thank you Pratik Doshi . I will try. Any coconut water would do or it has to be made specifically for plant tisse culture?
I prefer the fresh coconut water (brown ones) that you get in the supermarket. If not available, any packaged cocunut water will do.
Did not understand if you use the ready MS, one can buy, or you prepare the MS by stock solutions?
Because you pointed to the FeEDTa.
If you prepare this as stock solution together with CuSO4, then you have to cook it! Only then you get the right Fe-solution. You have to wait until the color is changing from light yellow tow dark yellow. Then let cool and fill up.
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