I am working with a nitrogen sensitive species trying to induce somatic embryogenesis. The textbooks I've gone through so far insist that casein hydrolysate - or another reduced nitrogen source - is crucial to initiating embryogenic callus. but many of the protocols I've read don't include CHL or any other nitrogen sources. So what's the truth here? Is this reagent crucial or not? I've ordered the casein hydrolysate just in case, but am trying to figure out if I'll even need it.
Dear Hailey Veltri unfortunately, or not, TC isn't like maths. Many factors can influence to obtain a result, or antoher, from biological (the species, the kind of explant...), passing through chemical (hormones, nutritive formulations, concentrations...) to physical/environmental (light intensity and regime used, temperature, protocol followed...) factors. Casein hydrolysate is an important source of amino acids, that has been proved its effectivity on somatic embryogenesys. Consider all the steps of your protocol, and conduct trustable trials to determine if CHL is suitable for your conditions, and scopes.
Regarding the omission of nitrogen sources I think it may be ammonium ions which is initially omitted in many research during the microcalli development and casein hydrolysate on the other hand is mixture of amino acid. But organogenesis is dependent on many factors like carbon source or hormones or additives so you have to standardize it. You can order MS without Ammonium salt it is available in duchefa.
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