How can we manipulate them without spreading this sticky liquid in our tools and media culture?
As it is the first time I work with milky sap plants, I appreciate all the experienced advices.
Micro propagation is propagation of plants through plant tissues.
Micropropagation is the artificial process of producing plants vegetatively through tissue culture or cell culture techniques. In this artificial process of propagation, plants are produced invitro by asexual means of reproduction or by vegetative propagation.
Plants can be produced both asexually i.e, via vegetative parts’ multiplication or sexually i.e., seed production. One of the means of asexual reproduction is by multiplying genetic replicas of plants that are referred to as clonal propagation wherein plants can be populated from a single individual through asexual means of reproduction.
Micro propagation is of various types depending of the type of tissue used in the process of asexual propagation.
Latex, a sticky emulsion produced by specialized cells called laticifers, is a crucial part of a plant’s defense system against herbivore and pathogens.
Taking into consideration the defense role of latex in plant development, it should not be surprising how complex and diverse the latex composition can be.
The collected latex is transferred to appropriate medium like agar for development of callus. Callus tissue lump can be cut for further propagation. Callus tissue after treatment is again transferred to agar gel with cytokinin and gibberellins in it for proper root and shoot development.
Fully grown saplings are transferred to soil.
Hi my dear,
For most of those plants, directly after cutting you can put in a clean water for about 30 minutes. After it, if you are using rooting hormon you put around the cutting places them you put the cutting in and appropriate substrate and follow it up.
Courage
You could try using different source tissues for the micropropagation. Seedlings may have little latex present and would be a good source of fresh growing cells. Seeds are also relatively easy to surface sterilize and grow aseptically.
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