Yes it is possible to analyse terpenes by GC-MS. Go ahead. I am attaching certain links and papers which might help you. Another advantage with you is that you have a purified standard also, so comparison can be done easily.

http://pubs.acs.org/doi/pdf/10.1021/ac60164a004

PFA pdf for GC anlaysis of terpenes in pine resins. Might help you.

PFA a guide to terpene analysis. Cannabis terpenes are used in this method. You can have a look and get some useful tips.

GUD LUCK

Regards

Thankyou Alok Nahata, I will read the papers with interest.

Yes, You can found many paper described methods about terpenes by GC-MS.

Hello, how colleg's says terpenes from c10 to c15 (and c20, c25 even) can be analyze using GC-MS with succses but:

1. nonpolar column is good but u must know many terpenes have similar retention time, even when u have slow temperature rate in oven. I recommend polar column also (when is possible) to compare results.Try using column with 30 x 0,2 x 200 or 60x0,25x250 maximum. With nonpolar column approche DB-5 (5% Ph groups) is better than DB-1 (100% pdms). How i mentioned many terpenes have similar retention index (retention time) and similiar mas spectrum (136, 123, etc.)

On polar column u can have vewry good results (there are polar column for high temperature like sol gel or other). The best chromatography is GCxGC but when u have possibility :). This is good lecture "Analysis of Terpenes in Resins and Varnishes by Capillary Gas Chromatography and Their Characterization by Pattern Recognition" , many papers describe analysis essential oils and related subjects using GC/MS. good luck.

Yes definetly as said above by Dr Nahata, Dr Dziadas. You can study all these terpenoids on HP-5, DB-5, DB-WAX, BP-20 on GC and GC-MS.

Gud luck

:)

Yes, it is possible to tedect this class of compounds by GC/MS.

However, as mentioned by other answers, the problem is the separatin and proper identification of your target compound.

But as you have the "reference" - why not trying it?

HPLC is a powerful technique, but GC/MS as well with quite higher peak resolution than possible even with UPHLC.

Please, check your standard at first carefully for the fragment pattern !

Hello Isidro, I agree with Dr Nahata and Dr Dziadas, GC-MS is the best choice for low molecular weight (< 500 dalton) and volatile compounds, such as your terpene. Their advices on the GC column to use should be right, however you must pay attention also to injection conditions (for instance liner type and temperature). Because of the chemical structure of jatrophone, you should not have any problem to see its molecular ion in EI together with an optimal fragmentation to compare with the standard.

Good luck Isidro.

Bye

Of course. Terpenoids and steroids can be analyzed by GC, depending of conditions of analysis. However you need check if your compound is stable at high temperature used in GC.

I agree with people above . It can be done and since you have purified standard you should be able to avoid problems with retention time. You can "spike" your sample (co-inject) or do two separate analyses one with sample and one with standard and compare - also with standard you can build calibration curve and quantify your samples.

It is possible to detect the jatrophone by using Ultra-2 Or Ultra-1 column in GC or GC-MS, also you can quantify from the area percentage.

Good Luck

Thanks to All of you. My doubt was based in the fact that Jatrophone has a MW of 312.

Usually GC-MS detectors are well enough in range of 40-45 to 450 or even more.

Yes, you can use GC-SM

Agree all above analysis. Based on the structure of jatrophone you specifically concerned, GC-MS should work properly, at least you can try and then improve the condition accordingly. Good luck

I have use GLC-MS analysis several times with very good results to analyze terpenoids in conifers.

I syggest to connect the instrument with computer having the right software to determine the quantities of your compounds.

You should also to try several kinds of columns as well temperature programms.

Hola isidro. Feliz 2013!. Respecto a tu pregunta: los terpenoides se pueden analizar en GC (como casi todos te han comentado). El asunto es que el compuesto que mencionas (CASNº 29444-03-9) es muy voluminoso y no se cual es su punto de ebullición (por ejemplo) entre otras propiedades físicas indispensables para predecir su comportamiento cromatográfico. No se si ese compuesto es termolabil. Primero deberías conocer esas propiedades y ver si es necesario derivatizar, o buscar otras alternativas analíticas. Saludos. Viviana

using ether extract ,then injection GC

I did many times for volatile oils

you can ckeck my articls

Using ether extract then injection GC-I have more than ten articles puplished in die nahrung,germany

Could you tell me the mobile phase in column chromatography of lupeol and beta sitosterol compounds. i am trying in ficus species.

Of course is it possible to detect and quantify terpenoid compounds by GC.

You can start by GC/FID. However, GC/MS is a preferred method. As you mentioned you have a purified standard, I wonder, why so many commands leads still quite similar answers.

IN principle, YES, you can.-

Thanks everybody for your answers. Until now the standards are being well separated, I have the mass spectra of the compounds (jatrophone and jatropholone), but unfortunately my library does not have registered them. I have the retention times too. Now I am searching for basic data of them as boiling point and volatility.

You didn't describe your GC/MS setup. Is it complete (with software), and running? Does it have a capillary column? If so, and it's a generic DB-1 of DB-5, the chances are good that you would be able to make a separation. Have you tried running your standard? Let me know if you need help tracking down references on these compounds, as there are a number that might help.

Hi,

I would like to analysis standard compounds geranyl pyrophosphate from GPP Lithium salt (http://www.sigmaaldrich.com/catalog/product/sigma/76532?lang=en®ion=US). Using Thermo Scientific™ Q Exactive™ GC Orbitrap™ MS. Has someone experience with analysis GPP using GC-MS? Could you please advice in this regards.

Thank you