Normally, we use oxidative agents like hyperchlorite to sterilize plant tissue to introduce into micropropagation. However, we are doing a plant that has high phenolic contents, and the oxidative agents caused severe browning. Is there any substitute disinfectants that is not of oxidative type to use in this case? We can't use mercury though because of its toxicity.
Dear Duy Minh Pham in my experience, I'd never succeeded using chemical antioxidants (citric acid, ascorbic acid, PVP,...); however, I've obtained great advantages selecting initiation materials.
Here in RG, you can find some papers describing how it's done.
Conference Paper Somatic Embryogenesis in Brazilian pine: establishment of bi...
Article Improved walnut mass micropropagation through the combined u...
Preprint Micropropagation of valuable walnut genotypes for timber pro...
Article Unraveling Factors Affecting Micropropagation of Four Persia...
Good luck!!!
Dear Duy Minh Pham I used ascorbic acid for date palm once and it was successful. depending on what explant you use the concentration and duration of treatment will be different. according to my experience the ascorbic acid only prevent browning during the sterilization but not at the initiation stage.
Dear Duy Minh Pham regarding the last part of your question, I forgot to answer you. Since phenolics are released to culture medium after wounding explants, the oxidative disinfectants might stimulate the oxidation of phenolics but it's not the main cause of detrimental effect of oxidative burst, at least in my opinion. Regarding HgCl, avoid its use as much as possible. It's a dangerous pollutant, and at least that you have a service to collect the wastes, don't use it.
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