I am a student conducting research for my course and I am having trouble in finding a pill for Ketoconazole. However, there are a lot of creams available in the pharmacies. Would it be possible to use that cream when testing against grown fungi on plates?
Ideally you want to know the exact concentration you are applying which is often not stated on commercial products. Also pills and creams often contain a mix of several antibiotics. Consider checking chemical suppliers for the pure compound.
For example:
https://dir.indiamart.com/impcat/ketoconazole-api.html
https://www.fishersci.co.uk/shop/products/ketoconazole-98-1/15321788
Using topical cream as the source of antibiotic for the disk diffusion method may not be the most reliable or standard approach. The disk diffusion method typically requires the use of standardized antibiotic disks containing specific concentrations of antibiotics. These disks are manufactured under controlled conditions to ensure accuracy and reproducibility of results.
Topical creams may contain various other ingredients besides the active antibiotic compound, which could interfere with the diffusion of the antibiotic into the agar medium or affect the results of the test. Additionally, the concentration of the antibiotic in a topical cream may not be suitable for the disk diffusion method.
If you need to perform the disk diffusion method, it's best to use commercially available antibiotic disks that are specifically designed for this purpose and have been validated for accuracy and reliability. If you're looking to test the efficacy of a topical cream, it's more appropriate to use other methods such as minimum inhibitory concentration (MIC) testing.
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