Hi, I want to use plant preservative mixture (PPM) solution to sterilize my explants (from ex vitro culture) and I have some questions: is it recommend/not to use the PPM sterilization solution more than once, like I use it to sterilize my explants and then I store the remaining used solution in the refrigerator to use it again in the next day/week? How many times can I reuse the solution? And how long can I store the PPM sterilization solution? Should I make the solution fresh before doing the initiation? Thank You in advance
Dear Hanggara Aji Sakti Mahambara Padma Negara I wouldn't suggest it: never subestimate microorganisms. Howerver, you've the answer in your hand: test it.
Plant preservative mixture (PPM) solution is commonly used for sterilizing plant tissue explants in tissue culture.
It is generally not recommended to reuse the PPM sterilization solution because it can lead to reduced effectiveness in sterilization over time. This is because the active ingredients in the PPM solution may degrade or become depleted with repeated use, leading to a lower concentration of the preservatives that are necessary for effective sterilization.
It is recommended to use freshly prepared PPM solution for each sterilization, and to avoid storing it for extended periods of time. The PPM solution can be stored at 4°C for a short period of time (a few days to a week), but it is best to prepare fresh solution as needed.
If you need to sterilize a large number of explants, you may need to prepare multiple batches of PPM solution or increase the volume of PPM solution used for each sterilization.
In summary, it is not recommended to reuse PPM sterilization solution, and it is best to prepare fresh solution for each sterilization to ensure maximum effectiveness.
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