Is it time for serious action on climate change?

30 November 2020 9,105 5 View

Why antenna reflection coefficient value changes when the frequency sweep range is changed?

Many times, what I observed is that the s11 of the designed antenna is changed when I change the frequency sweep in hfss. I can't understand, that why does the antenna response change. Please...

30 September 2020 4,129 2 View

Could someone explain the common cause of mismatching in reflection coefficient at higher frequencies in the measured result of antenna?

Many times the measured results of antenna matches with simulated but sometimes measured results do not match with simulated and sometimes measured results do not match at higher frequencies. Plz...

07 September 2020 3,550 14 View

DNA and RNA extraction from tissue sample ?

Is there a simple extraction process for both DNA of a bacteria and RNA of a virus from infected tissue sample without losing either DNA or RNA from the sample ???

08 June 2020 2,245 4 View

Which stain is used to see the deposition of oil or oil glands in anatomy section of leaf(transverse section) in cymbopogon?

Cymbopogon is an aromatic grass which produces essential oils as a secondary metabolite so to see the deposition of oil in leaves transverse section of intra and inter species can you suggest me...

28 August 2019 9,357 2 View

How to clone of Github NetSim project?

18 June 2019 1,289 2 View

How do I encrypt NetSim Payload encryption through MATLAB?

Instead of using the default encryption algorithms in NetSim, I want to use MATLAB to encrypt the packet payload of Netsim. This needs to be done for everypacket generated in NetSim. How can I do...

16 May 2019 1,886 1 View

What should be the strategy to increase pulses production in India?

How we can transform India from a pulse deficit country to pulse surplus state.

10 April 2019 4,639 3 View

How can poverty be reduced in future with the help of Agri-entrepreneurs?

Agripreneurship development is a key tool for poverty reduction. It has been proven effective for stimulating employment and economic growth in developing countries in Asia and Africa....

12 March 2019 6,261 3 View

What is the appropriate protocol for generation of Dendritic cells from mouse bone marrow..should it be GM-CSF+IL-4 or only GM-CSF?

Hi everyone, I am trying to generate Dendritic cells from mouse bone marrow where I am culturing the bone marrow cells with GM-CSF for 6 days and measuring the percent DCs generated on 6th day by...

07 March 2019 4,561 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

No title

28 February 2021 9,936 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View