Fifty milligram of methanol extract fraction were weighed separately and dissolved in 100 ml of methanol to get 500 μg/ml stock solution. From the stock solution how to prepare different concentration like (10, 20, 40, 80 or 160 μg/ml) or
10 mg of sample dissolved in 100 ml of methanol to get 100 μg/ml stock solution. From the stock solution how to prepare different concentration Like (2, 4, 6, 8 or 10 μg/ml)
please help with the calculation.
To make various concentrations (10 ug/ml, 20 ug/ml, 30 ug/ml) from a 500 ug/ml stock solution, you can follow these steps:
For a 10 ug/ml solution:
C1 = 500 ug/ml
V1 = ?
C2 = 10 ug/ml
V2 = 1000 ul (1 ml)
Substituting these values into the formula: 500 ug/ml x V1 = 10 ug/ml x 1000 ul
Solving for V1: V1 = (10 ug/ml x 1000 ul) / 500 ug/ml = 20 ul
To prepare a 10 ug/ml solution, take (1000 ul - 20 ul) = 1980 ul.
Then, add 20 ul of your stock solution (500 ug/ml) to 1980 ul of water or any desired solvent. This will result in a 10 ug/ml solution.
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