HOW to calculate pectinase enzyme activity as, we have values of OD @540 nm obtained from DNS method of different Ph and Temp? please let me know the calculation?
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I am determining the alpha amylase inhibition activity of my methanolic extracts by DNSA method. The problem is, my sample absorbance is higher than the negative control which made the final...
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We followed the NREL laboratory protocol to analyze reducing sugar after pretreatment of biomass via DNS method. But everytime we found the UV-Abs peak at 370 instead of 540nm. Suggestions are...
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The reducing sugars were estimated by DNS method.
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I measured reducing sugar using the DNS method, but when I removed the test tubes from the water bath after incubation and left them to cool, I noticed that the colour had vanished. Please solve...
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I learned DNS assay to find out the quantity of maltose in the sample. I realized the importance about the boiling water in this assay. But I got a several question after that. I don't know the...
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How to calculate pectinase enzyme activity as, we have values of OD @540 nm obtained from DNS method of different Ph and Temp of sub merged fermentation using wheat bran and citrus peel ? please...
15 August 2023 3,422 3 View
How can i prépare a pectine gélose to détect the pectinase activité in bacteria
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Is there a mimetic enzyme that can replace alkaline pectinase?
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Can I perform simultaneous pectinase, cutinase and cellulase assay using fruit peels??
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I already prepare the standard curve and read the absorbance reading for reducing sugar, then how to relate that with standard curve?
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