How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How I can extract pectinase from filamentus fungi? also get SDS-PAGE from it?

I am working on filamentus fungi, I culture it on broth culture and find the best time for extraction but still I cannot make sure that the way of extraction that I used is correct, actually I...

14 January 2021 4,150 3 View

How to calculate enzyme activity (for alpha amylase using DNS method) in excel sheet?

i have done amylase assay using DNS method. i want to calculate enzyme activity from absorbance in excel sheet. the standard curve prepared using maltose hydrate. please enlighten me how to...

02 July 2020 5,111 2 View

I feel difficulties to under stand the preparation of standards for alpha amylase assay. I has been upload some information kindly help me???

15 March 2020 5,872 3 View

What is the MWCO size of dialysis membrane used for glucose diffusion inhibitory assay by plant extracts?

I have conducted the above test for Water, methanol and ethanol extracts of herbal plant leaves. MWCO of the dialysis membrane I have used was 12-14KD. The problem I face was plant color also...

10 November 2019 9,794 2 View

Enzyme assay for pectinase, amylase and cellulase etc?

In different enzyme assay protocols like CMCase, pectinase, amylase, using 1 % or 0.5% substrate. In protocols they did not mention how substrate solution should be prepared whether it will be in...

07 August 2019 9,776 3 View

What is statistics of various classes of internet traffic?

hello. in many articles the web traffic behavior has been analyzed and some statistics of web traffic of a dataset such as request size, inter-arrival time, ... has been published. i need such...

06 July 2019 9,085 3 View

How to optimize production of enzymes using E. coli BL21(DE3)?

Hi, Previously i have used shake flask method to optimize production of pectinase and then i have done its stability analysis. But now i have to increase its production using fermenter tank and...

04 May 2019 4,032 1 View

Is the color density of the DNS reagent affected by the type of buffer used in the experiment?

Is the color density of the DNS reagent affected by the type of buffer used in the experiment?

01 February 2019 3,491 3 View

Is there any public available datasets for real APT attack scenarios?

Dear researchers, I am looking for some suitable datasets on real Advanced Persistent Threat-based Attacks (APTs) (especially those reported in...

11 December 2018 8,932 0 View

Doubly periodic conditions (two-directions) for turbulent rectangular channel flow using LES in ANSYS Fluent?

I need a periodic BC where mass flow rate is specified to model fully developed flow, and another periodic BC with no pressure drop. However, once I define the mass flow rate for one periodic BC,...

18 November 2018 4,845 7 View