I've already used some human cell lines for in vitro assays, but I would like to inject cancer cells in C57Bl6 mice (not nude).
- If your point is to inject immune-competent mice, the 4T1 model works very well, but 4T1 cells are derived from the BALB/c strain (http://www2.healthsci.tufts.edu/saif/4T1.htm).
- A C57Bl6-based alternative is the EO771 model. I have no direct experience with that, but it looks closer to what you are looking for (Kanda T et al., Cancer Res. 2009 Dec 15;69(24):9354-9).
- If you are interested in monitoring the interactions with the host immune system at different stages of tumor progression, the best model would be the FVB/N-Tg(MMTVneu)202Mul/J transgenic strain (http://jaxmice.jax.org/strain/002376.html).
- If your point is to inject immune-competent mice, the 4T1 model works very well, but 4T1 cells are derived from the BALB/c strain (http://www2.healthsci.tufts.edu/saif/4T1.htm).
- A C57Bl6-based alternative is the EO771 model. I have no direct experience with that, but it looks closer to what you are looking for (Kanda T et al., Cancer Res. 2009 Dec 15;69(24):9354-9).
- If you are interested in monitoring the interactions with the host immune system at different stages of tumor progression, the best model would be the FVB/N-Tg(MMTVneu)202Mul/J transgenic strain (http://jaxmice.jax.org/strain/002376.html).
E0771 is the one we use in C57BL/6 mice. We inject 3 x 10exp6 cells sc and this forms nicely growing tumors.
Yes, I also used E0771 cell in C57BL/6 mice. In our hands they form tumors only with matrigel, but we used about 1 millions cells per mouse
Hi Olivia,
It would help me a lot if you could explain briefly the purpose of your experiment. And perhaps even that of Jo and Vladimir, who answered your request.
Regards,
Peter Duesberg
there is the MMtV-Pymt line. It has been established by Dr Scott Abrams and published here http://www.cell.com/retrieve/pii/S0092867412006435
Hi, you can use E0771 for breast cancer and B16-F10 for melanoma studies. The mouse strain name is C57B/6.
Hi Olivia, I have used E0771 cells, injected s.c. in the right flank. They grew well in vendor-purchased C57BL/6 mice but do start ulcerating when they became large. Implantation number was about 1 million cells/mouse and did not require matrigel. PMID: 19118005
Hi, Olivia:
Ideally you inject into the mammary fat pad. Tumors grow differently in differently anatomic regions. If you are interested in breast cancer biology, then orthotopic implantation in second best to primary tumors such as the ones mentioned by Leonardo or Mahendra.
Hi Which medium is better for maintenance E0771 cell line? RPMI 1640 with 10mM HEPES and 10% serum or DMEM with 20% serum? I appreciate any help.
I'm using RPMI, and they grow super fast. I never tried DMEM. Good Luck!But for in vivo fat pad injection we must use matrigel.
Hopefully useful, Eo771 in vivo:
http://link.springer.com/article/10.1007%2FBF01810679#page-1
http://cancerres.aacrjournals.org/content/69/24/9354.full
http://jem.rupress.org/content/210/3/563/F3.expansion.html
Olivia, did you get finally the E0771 cells?. If yes, it would be possible to get an aliquot?
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