07 December 2014 5 4K Report

In normal plasmid isolation kits The columns always get exhausted way before solutions. So if any company provides only column, kindly let me know

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How do I verify the Harmonic response simulation? What kind of tests can I do except vibration test?

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12 October 2023 8,168 2 View

How to identify the regulations of river flow?

is it possible to identify the regulation or what is the probability that the river flow is regulated, based on only observed streamflow data and/or meteorological data such as precipitation? If...

08 February 2023 2,892 3 View

Why does religation problem persists in Vector backbone while digesting with two different restriction enzymes?

Our aim is to transfer gene from bacterial plasmid(pET22b) into mammalian expression vector pACGFP1-N1, i have decided restriction sites available in pACGFP1-N1 vector MCS but while doing...

24 January 2023 446 3 View

How to prevent pellet formation in aspergillus oryzae ?

I am trying to inoculate transformed fungal colonies of aspergillus oryzae from drop out media using tooth pick. I am incoulating them in 10ml YPD media with the help of toothpeak. I am getting...

15 July 2022 10,000 3 View

What are the tools to analyze protein network other than STRING of more then 2000 proteins?

We have a protein profile of around 4000 proteins, and we tried Cytoscape since STRING has limitation of 2000 maximum , Cytoscape slowed down the system and taking more time, i also tried GEPHI...

14 February 2022 2,083 0 View

What is the alternative to Glucanex lysing enzyme ?

Sigma lysing enzyme L1412 has been discontinued. Is there a known commercially available alternative to this ?

19 April 2021 4,726 4 View

How can we extract digital elevation data from Google Earth?

Is there any option or methodology to download Google Earth's Digital Elevation Data? Is there any tools or software available to extract the digital elevation model (DEM) of Google Earth?

12 April 2021 1,588 3 View

Error in QGIS: The following layers were not correctly generated.. is there any solution for this?

While performing "Terrain Analysis" tool such as "Strahler Order, channel network and drainage basin" of SAGA in QGIS, I often found the error message as result which says "The following layers...

15 March 2021 8,307 7 View

Journals with low APC or no APC?

Do you know any journals in the area of medicine/pediatrics/nutrition that publish protocols of systematic reviews? No APC or Low APC

04 January 2021 655 14 View

What is the good Molprobity score for a protein homology model??

I build a homology model for a protein which show 44% sequence identity with its template and molprobity score is 1.59

27 December 2019 3,416 4 View

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