Nanxi Wang

6 Questions 28 Answers 0 Followers

Questions related from Nanxi Wang

Why does HEK293T detacht after transfection?

Hi, I was using costar 3516 6-well plate to culture HEK293T and reached the confluency 80-90%. Then I mix 1ug plasmid DNA with 2.5uL Plus reagent in 500uL DMEM. 10uL lipofectamine LTX reagent with...

01 October 2015 5,860 13 View

Any suggestions on PNL43 construct transformation efficiency being very low?

Hi, I have been using thermo T4 ligase to do ligation for pNL43 constructs. I followed the protocol of life tech to do chemical transformation into STBL2. I used Genehog (DH10B derivative) as...

20 April 2015 547 7 View

Are reverse transcriptase and integrase necessary for HIV-1 packaging in 293T cell and TZM-bl assay?

Hi, I am trying to mutate HIV-1 reverse transcriptase and integrase to make dysfunction phenotypes. I am wondering are they necessary for TZM-bl cell infection assay (X-gal or luc)? Another...

08 April 2015 3,519 2 View

Promoter and terminator of Fum 9 and Fum10 (Fusarium verticillioides)?

I am trying to predict the promoter and terminator of Fum 9 and Fum10 (Fusarium verticillioides) with a software. I include 500bp upstream of ORF. Different promoter prediction softwares were...

08 December 2014 6,360 6 View

Does TZM-bl package and release HIV-1 virus particles?

I learned that people normally use 293T or 293FT to package HIV-1 and then infect TZM-bl. Can HIV-1 get released from TZM-bl and then infect other TZM-bl cells? I read about using pseudo virus to...

02 December 2014 4,107 18 View

Is Zyppy miniprep kit reliable？

Since last summer plasmids sequencing always fails in my lab. We use Zyppy miniprep kit. The other lab using qiagen miniprep kit never have problems. Now we have to PCR our fragment first, and...

26 September 2014 3,922 3 View