Nikunj Daga

9 Questions 10 Answers 0 Followers

Questions related from Nikunj Daga

How to prevent pellet formation in aspergillus oryzae ?

I am trying to inoculate transformed fungal colonies of aspergillus oryzae from drop out media using tooth pick. I am incoulating them in 10ml YPD media with the help of toothpeak. I am getting...

16 July 2022 9,982 3 View

What is the alternative to Glucanex lysing enzyme ?

Sigma lysing enzyme L1412 has been discontinued. Is there a known commercially available alternative to this ?

20 April 2021 4,699 4 View

Can anyone suggest a good sporulating media (solid/liquid) for trichoderma reesei ?

I need to attain high volumes of trichderma spores to be used as inculum for pilot scale fermentation.. The ususal method scaling up with broth , like PDB, is not desirable for industries. I need...

08 July 2019 7,888 21 View

What is the most convenient way of growing anaerobic bacteria in shake flasks?

My organism is an obligate anearobe but aerotolerant, Hence a certain degree of anaerobic conditions has to be maintained. I am well aware of the medium requirements of my organism and the basic...

15 October 2015 7,190 3 View

How to dissolve D-biotin in water or any other liquid formulation to a concentration of 4gram/liter ?

I want to use it for one of my media composition. since the solubility of biotin in very less, reported to be 0.02%, I cannot use it for pilot scale media composition as filter sterilization would...

26 September 2015 3,003 8 View

How do I ascertain if the bacteria is sporulating and then quantify the number of spores?

I am working on a bacillus species and ignorant of any method that would help me achieve this. Thanks

26 May 2015 6,581 4 View

What are most frequently used chemical mutants for trichoderma reesei for extracellular enzyme production?

I am working on industrial enzymes and want to overexpress native enzymes

27 January 2015 4,497 4 View

Are silica gel columns for miniprep plasmid isolation available commercially ?

In normal plasmid isolation kits The columns always get exhausted way before solutions. So if any company provides only column, kindly let me know

07 December 2014 4,431 5 View

How can I differentiate bacterial contamination in fungal wet mount microscopically?

Basically I work on solid state fermentation of fungus and find it really difficult to confirm if my seed cultures are pure.

07 December 2014 6,011 7 View