Can anyone help me identify the proteins from the mass spectra?

In order to see the binding of a drug to proteins of interest, I used DARTS assay. In the 1D gel, one lane was loaded with crude protein sample and the other lane was loaded with crude protein...

01 February 2018 8,046 4 View

Labs in India where protein-drug interaction studies are possible?

I would like to check the physical interaction between a protein and drug in vitro. Can anyone suggest the best method to study protein-drug interaction and where in India I can outsource this...

05 June 2017 2,453 2 View

Which is the most cost effective way to study Protein-drug interaction in vitro?

I need to analyze the interaction/binding of a drug to a protein. Protein overexpression and purification seem to be every expensive. Is there any way I can study protein-drug binding/interaction...

03 April 2017 4,659 3 View

What the possible type of contamination is this?

The following (picture) unknown organism has contaminated our SH-SY5Y cell line, any idea what it is? They are only there in the flasks with cells, not in flasks with culture media alone.

08 September 2016 1,691 7 View

What happens to Blood Brain Barrier after cerebral ischemic stroke?

Can stroke destroy the integrity of Blood Brain Barrier completely? If so, for how long? During the compromised period molecules with any molecular weight can cross BBB?

05 June 2016 1,936 3 View

How to do a Protein-Drug-Protein MD Simulation?

I have Protein A, Protein B and a Drug (small molecule). Protein B is an enzyme which can bind to protein A and can hydroxylate it. During docking study, my drug bound to Protein A binding site of...

04 May 2016 2,739 4 View

What should be the SH-SY5Y cell density for doing MTT assay after Oxygen Glucose deprivation?

11 December 2014 1,467 6 View

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Does error (difference of less than 3 degree Celsius) in annealing temperature have any role in getting a higher Cq/Ct?

08 September 2014 9,325 13 View

Can anyone help with RNA isolation from Neuroblastoma cells?

07 August 2014 4,350 4 View

Meta-analysis: Data analysis part?

Currently I am working on a systematic review with meta-analysis. I will be writing review about the neuroprotective efficacy on a drug. From the extracted papers I collected infarct volume and...

07 August 2014 3,576 12 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View