12 Questions 16 Answers 0 Followers
Questions related from Charles K Davis
In order to see the binding of a drug to proteins of interest, I used DARTS assay. In the 1D gel, one lane was loaded with crude protein sample and the other lane was loaded with crude protein...
02 February 2018 8,097 4 View
I would like to check the physical interaction between a protein and drug in vitro. Can anyone suggest the best method to study protein-drug interaction and where in India I can outsource this...
06 June 2017 2,566 2 View
I need to analyze the interaction/binding of a drug to a protein. Protein overexpression and purification seem to be every expensive. Is there any way I can study protein-drug binding/interaction...
04 April 2017 4,715 3 View
My lab would like to procure SK-N-SH or SH-SY5Y cell line. Thanks in advance.
11 November 2016 7,974 2 View
The following (picture) unknown organism has contaminated our SH-SY5Y cell line, any idea what it is? They are only there in the flasks with cells, not in flasks with culture media alone.
09 September 2016 1,745 7 View
Can stroke destroy the integrity of Blood Brain Barrier completely? If so, for how long? During the compromised period molecules with any molecular weight can cross BBB?
06 June 2016 2,003 3 View
I have Protein A, Protein B and a Drug (small molecule). Protein B is an enzyme which can bind to protein A and can hydroxylate it. During docking study, my drug bound to Protein A binding site of...
05 May 2016 2,797 4 View
I am planning to do MTT assay after Oxygen Glucose deprivation (OGD) and drug treatment for SH-SY5Y cell line. What will be the appropriate cell density per well (96 well plate) for seeding?....
12 December 2014 1,521 6 View
Does error (difference of less than 3 degree Celsius) in annealing temperature have any role in getting a higher Cq/Ct?
09 September 2014 9,375 13 View
Currently I am working on a systematic review with meta-analysis. I will be writing review about the neuroprotective efficacy on a drug. From the extracted papers I collected infarct volume and...
08 August 2014 3,708 12 View
Any one using "innuPREP DNA/RNA Mini Kit" from Analytik Jena fro RNA isolation? I have a problem, tried isolating RNA from SH-SY5Y cells but failing at the first step itself. Not getting any...
08 August 2014 4,401 4 View
I have b-actin as my reference gene and 9 other genes as targets. B-actin primers have a annealing temperature of 52 degree and few genes have got annealing temperature (Ta) 55 and rest have 58.
08 August 2014 3,477 6 View
