Can anyone help me identify the proteins from the mass spectra?

In order to see the binding of a drug to proteins of interest, I used DARTS assay. In the 1D gel, one lane was loaded with crude protein sample and the other lane was loaded with crude protein...

01 February 2018 8,046 4 View

Labs in India where protein-drug interaction studies are possible?

I would like to check the physical interaction between a protein and drug in vitro. Can anyone suggest the best method to study protein-drug interaction and where in India I can outsource this...

05 June 2017 2,453 2 View

Which is the most cost effective way to study Protein-drug interaction in vitro?

I need to analyze the interaction/binding of a drug to a protein. Protein overexpression and purification seem to be every expensive. Is there any way I can study protein-drug binding/interaction...

03 April 2017 4,659 3 View

Anyone would like to share SK-N-SH or SH-SY5Y cell line in India?

My lab would like to procure SK-N-SH or SH-SY5Y cell line. Thanks in advance.

10 November 2016 7,921 2 View

What the possible type of contamination is this?

The following (picture) unknown organism has contaminated our SH-SY5Y cell line, any idea what it is? They are only there in the flasks with cells, not in flasks with culture media alone.

08 September 2016 1,691 7 View

What happens to Blood Brain Barrier after cerebral ischemic stroke?

Can stroke destroy the integrity of Blood Brain Barrier completely? If so, for how long? During the compromised period molecules with any molecular weight can cross BBB?

05 June 2016 1,936 3 View

How to do a Protein-Drug-Protein MD Simulation?

I have Protein A, Protein B and a Drug (small molecule). Protein B is an enzyme which can bind to protein A and can hydroxylate it. During docking study, my drug bound to Protein A binding site of...

04 May 2016 2,739 4 View

No title

Does error (difference of less than 3 degree Celsius) in annealing temperature have any role in getting a higher Cq/Ct?

08 September 2014 9,325 13 View

Can anyone help with RNA isolation from Neuroblastoma cells?

07 August 2014 4,350 4 View

Meta-analysis: Data analysis part?

Currently I am working on a systematic review with meta-analysis. I will be writing review about the neuroprotective efficacy on a drug. From the extracted papers I collected infarct volume and...

07 August 2014 3,576 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View