Hi,
I am initiating callus from hypocotyl in Catharanthus roseus, using MS media with 1mg/l of both NAA and BAP. The callus looks good. My concern is that the color of the callus is dark green and although it is not generating any shoots or roots at the moment (after about 20 days), I am afraid it will start to regenerate. I don't want that and I need to keep them as they are. Should I make any changes? Is it normal for Catharanthus roseus to have dark green callus?
Any thought or suggestion is appreciated.