Mohammad Alimohammadi

9 Questions 6 Answers 0 Followers

Questions related from Mohammad Alimohammadi

Where to find antibodies without Sodium Azide?

I was wondering if anybody knows about a source that we can order commercial antibodies without Sodium Azide? I understand that we can use dialysis to remove that but I wanted to know if ordering...

26 March 2018 610 3 View

How can I determine if tagging a protein with fluorescent tag affects protein functionality?

I am planning to tag a protein with mCherry using CRISPR/CAS9 method. I need to know if adding the mCherry seq. on 3'UTR can affect the functionality of protein or if I should add the mCherry...

10 October 2017 9,594 7 View

Is there a database that lists all or most of the academic research labs in the U.S.?

Hi, I am looking for a comprehensive list of research labs in the u.s. Maybe categorized by area of research or by the name of the universities? I understand that this would be a very long list...

14 March 2017 6,867 1 View

How to design sgRNA from antisence sequence?

I am trying to design the oligonucleotides used for making sgRNA plasmid used in CRISPR.The online tool that I used (https://chopchop.rc.fas.harvard.edu/) gives me the suggestion for choosing the...

25 October 2016 574 5 View

How to induce autophagy in hela cells?

I need to induce autophagy in hela cells for my experiment. Theoretically it is simple. 4 hours of starvation in EBSS medium should do the trick according to literature. However, after the...

31 July 2016 9,273 8 View

Anyone familiar with catharanthus roseus callus formation?

Hi, I am initiating callus from hypocotyl in Catharanthus roseus, using MS media with 1mg/l of both NAA and BAP. The callus looks good. My concern is that the color of the callus is dark green and...

21 October 2015 8,884 2 View

Why do you think more and more recent graduates and post-docs consider a job outside academia?

Finding a job in academia is already very tough for new graduates considering the funding limitations and extreme competition. What are the reasons in your opinion? What are the consequences of...

05 January 2014 6,348 4 View

Is it right to patent science? Especially in the fields that directly affect human life?

What are the advantages and disadvantages of patenting science? Can patenting be dangerous for science? Your opinion?

02 November 2013 7,327 7 View

What book do you recommend explaining molecular biology to public audience or entry-level biology students?

I'm looking for a book explaining molecular biology in a language which is understandable and exciting for non-biology majors or freshman college students. The idea is to make such people...

23 December 2012 4,568 13 View