Usually I use the calcein or the MTT, but my present experiments consist in determining the quantity of mitochondrial ROS in my stressed cells and my results of cellular viability seem not logical. I 'm looking for an other marker of cellular viability. Thanks in advance for your help
Dear Nesrine,
Check this link out if it helps:
https://fi.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/cell-viability/
Dear Nesrine,
Please check the following:
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/probes-for-reactive-oxygen-species-including-nitric-oxide/generating-and-detecting-reactive-oxygen-species.html
Generating and Detecting Reactive Oxygen Species—Section 18.2
Chapter 18—Probes for Reactive Oxygen Species, Including Nitric Oxide
http://www.cellsignal.com/browse/?Ntt=Mitochondrial&gclid=Cj0KEQiAv5-zBRCAzfWGu-2jo70BEiQAj_F8oNr6hMniaRKLUdttRy6vlSJOIGEtjsaCLnuPEUdvTOgaArpd8P8HAQ
Hoping this will be helpful,
Rafik
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