Nesrine Abboud

3 Questions 3 Answers 0 Followers

Questions related from Nesrine Abboud

Any idea about cellular viability markers, which are not influenced by the mitochondrial activity and by the dynamics of the cellular membrane?

Usually I use the calcein or the MTT, but my present experiments consist in determining the quantity of mitochondrial ROS in my stressed cells and my results of cellular viability seem not...

09 December 2015 9,625 3 View

How can we make a gene correction of LRRK2 mutation on iPSC reprogrammed from fibroblasts of a parkinsonian patient ?

I should correct the LRRK2 mutation on my cells. But I hope to know is it better to do the correction on fibroblasts (before reprogramming them on iPSC) or on iPSC (after reprogramming)? And if...

12 November 2014 9,036 1 View

What is the best way to inhibit a gene expression: siRNA or shRNA?

I have to inhibit a gene expression within mouse embryonic stem cells for a minimum duration of 15 days.

05 December 2013 1,172 2 View