Any advice on the peritoneal macrophage death observed the next day?

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Indole 3 propionic acid supplement to mice?

Why mice get aversion towards IPA supplemented in water?

08 September 2017 3,055 3 View

Griess in Gut Exudate?

Is it possible to do NO (nitric oxide) measurement (using Griess) in Gut exudate in mice? Would it impact the NO release by peritoneal macrophages? Suggestions are welcome.

02 March 2017 6,654 0 View

Chemical Ageing Mediators in Mice?

I am interested to carryout ageing research using mice as the tool. We have genetic knockouts for the protein of interest. I am curious to know if there are any ways (chemically or...

10 November 2016 3,601 2 View

Endothelium Derived Hyperpolarization Factors and significance?

I am interested to know if EDHF a primordial factor for relaxation? What are the physiological relevance and sources?Does EDHF observed in Conduit artery provides any significant impact in...

03 April 2016 1,102 15 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

31 December 2015 8,825 1 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

31 December 2015 9,812 4 View

Can the inguinal lymph nodes be pooled for FACS analysis in mice? Is it acceptable?

I am planning to analyse the T-cell population in lymph nodes. Is it okay that I could pool the 2 inguinal lymph nodes after euthanizing the mice and then stain and analyze? Or do I have to do...

10 November 2015 6,331 4 View

Vascular smooth muscle cell (VSMC) Murine.

How long should VSMC's murine be starved to study the switch on of mediators like erk1/2 phospo etc.? I tried twenty-four hours, but there were no major changes.

01 February 2013 5,634 0 View

Custom media ordering?

I am interested to buy custom media for my cell culture. Any recommendation about vendors for Universities in Canada? Thanks

01 January 1970 8,080 2 View

Cardiomyocyte phenotype in mouse

Recently, i got intrigued in mouse cardiomycytes - i am curious to know the different cardimyocyte phenotypes that exist in mouse heart (2-3months old) - Are there specific markers for...

01 January 1970 7,783 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View