Indole 3 propionic acid supplement to mice?

08 September 2017 2,980 3 View

Griess in Gut Exudate?

Is it possible to do NO (nitric oxide) measurement (using Griess) in Gut exudate in mice? Would it impact the NO release by peritoneal macrophages? Suggestions are welcome.

02 March 2017 6,579 0 View

Chemical Ageing Mediators in Mice?

I am interested to carryout ageing research using mice as the tool. We have genetic knockouts for the protein of interest. I am curious to know if there are any ways (chemically or...

10 November 2016 3,540 2 View

Endothelium Derived Hyperpolarization Factors and significance?

I am interested to know if EDHF a primordial factor for relaxation? What are the physiological relevance and sources?Does EDHF observed in Conduit artery provides any significant impact in...

03 April 2016 956 15 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

31 December 2015 9,749 4 View

Can the inguinal lymph nodes be pooled for FACS analysis in mice? Is it acceptable?

I am planning to analyse the T-cell population in lymph nodes. Is it okay that I could pool the 2 inguinal lymph nodes after euthanizing the mice and then stain and analyze? Or do I have to do...

10 November 2015 6,266 4 View

Any advice on the peritoneal macrophage death observed the next day?

04 May 2015 8,642 4 View

Vascular smooth muscle cell (VSMC) Murine.

How long should VSMC's murine be starved to study the switch on of mediators like erk1/2 phospo etc.? I tried twenty-four hours, but there were no major changes.

01 February 2013 5,558 0 View

Custom media ordering?

I am interested to buy custom media for my cell culture. Any recommendation about vendors for Universities in Canada? Thanks

01 January 1970 8,021 2 View

Cardiomyocyte phenotype in mouse

Recently, i got intrigued in mouse cardiomycytes - i am curious to know the different cardimyocyte phenotypes that exist in mouse heart (2-3months old) - Are there specific markers for...

01 January 1970 7,719 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View