Is it possible to do NO (nitric oxide) measurement (using Griess) in Gut exudate in mice? Would it impact the NO release by peritoneal macrophages?
Suggestions are welcome.
Why mice get aversion towards IPA supplemented in water?
08 September 2017 3,055 3 View
I am interested to carryout ageing research using mice as the tool. We have genetic knockouts for the protein of interest. I am curious to know if there are any ways (chemically or...
10 November 2016 3,601 2 View
I am interested to know if EDHF a primordial factor for relaxation? What are the physiological relevance and sources?Does EDHF observed in Conduit artery provides any significant impact in...
03 April 2016 1,102 15 View
I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...
31 December 2015 8,825 1 View
31 December 2015 9,812 4 View
I am planning to analyse the T-cell population in lymph nodes. Is it okay that I could pool the 2 inguinal lymph nodes after euthanizing the mice and then stain and analyze? Or do I have to do...
10 November 2015 6,331 4 View
Just a curious question, this was faced by my friend. He isolated peritoneal macrophages and found them dying the next day after plating. is it common?
04 May 2015 8,701 4 View
How long should VSMC's murine be starved to study the switch on of mediators like erk1/2 phospo etc.? I tried twenty-four hours, but there were no major changes.
01 February 2013 5,634 0 View
I am interested to buy custom media for my cell culture. Any recommendation about vendors for Universities in Canada? Thanks
01 January 1970 8,080 2 View
Recently, i got intrigued in mouse cardiomycytes - i am curious to know the different cardimyocyte phenotypes that exist in mouse heart (2-3months old) - Are there specific markers for...
01 January 1970 7,783 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
or can you recommend articles showing the differential effect of LPS in a time dependant manner?
28 July 2024 2,547 1 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
This research question explores the complex interactions between oral microbes and the oral sensory system. The diversity and function of oral microbial communities play an important role in oral...
09 July 2024 3,601 0 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
01 July 2024 7,007 1 View
Hello, all I am currently extracting LPS from E. coli MG1655 and its mutant strains. I followed several different protocols (phenol methods or phenol+diethy ether methods), but I couldn't see...
25 June 2024 7,568 1 View
I am working on a project that requires stimulating RAW 264.7 NF-kB macrophage and readout my signal by luciferase assay. Although all concentrations tested (10, 50, 100ng/mL) after 24 hours...
16 June 2024 2,885 3 View
Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...
15 June 2024 3,208 4 View