Indole 3 propionic acid supplement to mice?

08 September 2017 2,980 3 View

Griess in Gut Exudate?

Is it possible to do NO (nitric oxide) measurement (using Griess) in Gut exudate in mice? Would it impact the NO release by peritoneal macrophages? Suggestions are welcome.

02 March 2017 6,579 0 View

Chemical Ageing Mediators in Mice?

I am interested to carryout ageing research using mice as the tool. We have genetic knockouts for the protein of interest. I am curious to know if there are any ways (chemically or...

10 November 2016 3,540 2 View

Endothelium Derived Hyperpolarization Factors and significance?

I am interested to know if EDHF a primordial factor for relaxation? What are the physiological relevance and sources?Does EDHF observed in Conduit artery provides any significant impact in...

03 April 2016 956 15 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

31 December 2015 8,763 1 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

31 December 2015 9,749 4 View

Can the inguinal lymph nodes be pooled for FACS analysis in mice? Is it acceptable?

I am planning to analyse the T-cell population in lymph nodes. Is it okay that I could pool the 2 inguinal lymph nodes after euthanizing the mice and then stain and analyze? Or do I have to do...

10 November 2015 6,266 4 View

Any advice on the peritoneal macrophage death observed the next day?

04 May 2015 8,642 4 View

Vascular smooth muscle cell (VSMC) Murine.

How long should VSMC's murine be starved to study the switch on of mediators like erk1/2 phospo etc.? I tried twenty-four hours, but there were no major changes.

01 February 2013 5,558 0 View

Custom media ordering?

I am interested to buy custom media for my cell culture. Any recommendation about vendors for Universities in Canada? Thanks

01 January 1970 8,021 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View