Indole 3 propionic acid supplement to mice?

08 September 2017 2,980 3 View

Griess in Gut Exudate?

Is it possible to do NO (nitric oxide) measurement (using Griess) in Gut exudate in mice? Would it impact the NO release by peritoneal macrophages? Suggestions are welcome.

02 March 2017 6,579 0 View

Endothelium Derived Hyperpolarization Factors and significance?

I am interested to know if EDHF a primordial factor for relaxation? What are the physiological relevance and sources?Does EDHF observed in Conduit artery provides any significant impact in...

03 April 2016 956 15 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

31 December 2015 8,763 1 View

Are soluble proteins active or inactive?

I am curious to know whether soluble proteins are active in circulation? or they become active after entering the extracellular space? I know it varies from protein to protein but would like to...

31 December 2015 9,749 4 View

Can the inguinal lymph nodes be pooled for FACS analysis in mice? Is it acceptable?

I am planning to analyse the T-cell population in lymph nodes. Is it okay that I could pool the 2 inguinal lymph nodes after euthanizing the mice and then stain and analyze? Or do I have to do...

10 November 2015 6,266 4 View

Any advice on the peritoneal macrophage death observed the next day?

04 May 2015 8,642 4 View

Vascular smooth muscle cell (VSMC) Murine.

How long should VSMC's murine be starved to study the switch on of mediators like erk1/2 phospo etc.? I tried twenty-four hours, but there were no major changes.

01 February 2013 5,558 0 View

Custom media ordering?

I am interested to buy custom media for my cell culture. Any recommendation about vendors for Universities in Canada? Thanks

01 January 1970 8,021 2 View

Cardiomyocyte phenotype in mouse

Recently, i got intrigued in mouse cardiomycytes - i am curious to know the different cardimyocyte phenotypes that exist in mouse heart (2-3months old) - Are there specific markers for...

01 January 1970 7,719 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View