Hi all,
I have been using YPDA from some time now to grow yeast, but I haven't been getting yeast growth in 3-4 days. The colonies that appear are tiny and when I am doing head to head interaction studies, they grow poorly on SD-alth x-a-gal media. I have stopped using YPD because the yeast growth in SD-alth is so not good too. Please let me know what I can do. I had only once had very good growth on YPDA with 3-4mm size yeast colonies, but the ones that are growing now are slow and very small, even though stock is same!
I don't think that the problem is the medium. As you said, the strains grew fine in the YPD before and they also are not growing well in your SD medium. So it probably is not that YPD is a problem but rather some systemic problem in the lab. First I would try to see whether it is specific to these strains or are all yeast strains not growing well. I would also look to see if there is anything that recently changed, a new batch of one of the reagents used in the medium, or perhaps the lab water is no longer as high quality as it was before. Or if you are using glassware and not disposable plastic ware, there might be a system problem with rinsing the glassware. Look through these thoughts and you should be able to come up with some approaches to troubleshoot the issue.
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