QUERY REGARDING acdS gene (ACC deaminase encoding gene) Amplification?

08 November 2020 3,249 2 View

How to scale an image whose scaling details are unknown?

I want to know how to add scale bar to a histopathological image using ImageJ whose image scale was not recorded at the time of taking the image.I got to know from the technicians that 1micron is...

03 November 2020 10,009 3 View

How to do scoring for fat droplets in Histopathological sections?

I want to do scoring for macrovesicles and microvesiclular fat diposits in Histopathological Liver sections. Can anyone suggest me a software and discuss a suitable protocol to achieve this. I...

18 October 2020 9,727 2 View

Significant value post Bonferroni correction?

Hi I did Friedman's RM ANOVA and found data is significant.Then Wilcoxon posthoc analysis is showing significant differences, p=0.001.But Bonferroni adjusted significance is bringing p value to...

07 October 2020 1,262 3 View

How to remove background and gridlines of graph in R?

I am trying to remove the background & gridlines from my Violin plot drawn by R. Please let me know the script/method. Also please let me know method to move the axis title n graph title in R....

07 October 2020 391 4 View

How to underatand whether to consider Linear, Quadratic or Cubic regression equation?

Hi I want to understand the relationship between two variables using correlation and regression. I performed Normality test and my data is parametric.After doing scatter plot analysis I observed...

29 September 2020 9,533 10 View

Is Repeated Measure Analysis for a Non-parametric data possible?

I want to do a Repeated Measure Analysis for data for three time points. But I can't do One Way RM ANOVA as the data is non-parametric, as is elucidated by Shapiro WIlk's Test for Normaility and...

02 September 2020 2,764 10 View

Which software other than WAMIT (I don't have an access to WAMIT) can be used to calculate added mass matrix of an AUV?

Im trying to find dynamics of underwater vehicle for which i need to get the added mass. I want to use computational approach for finding added mass. In literature people has used WAMIT but I dont...

17 March 2019 1,908 2 View

For blurring an image in frequency domain, can I multiply the N*N size image directly with the N*N gaussian filter matrix and take it's IFFT?

I am trying to implement gaussian filters in python in frequency domain. I have taken my input image in an array of size N*N, when i multiply this with the gaussian filter in frequency domain...

05 November 2018 4,399 1 View

How to get rid of background colour of hemolysate in SOD estimation by Pyrogallol autooxidation method??

I have tried to estimate SOD from blood hemolysate by Pyragallol autooxidation method of Marland n Markland. I have followed the following steps : BLANK: 50mM Tris EDTA Buffer....

23 April 2018 8,111 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View